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RNA Extraction and Real-Time Polymerase Chain Reaction Assay Total RNA was extracted from frozen jejunum samples with TRIzol reagent (Sigma-Aldrich), and was used to synthesize cDNA with Prime-Script RT reagent kit (Takara, Dalian, China) according to the manufacturers instructions

An average relative signal of 0.03 was estimated, comparing glutathionylated peptides to alkylated peptides

The concentration of glutathione in the sample was calculated by a standard curve that was prepared utilizing solutions of glutathione with known concentrations

S deficiency activates gene expression of BGLU28 and BGLU30 (Article 11, 15, 16)