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The resulting spectral changes allowed us to monitor the balance between reduced (roGFP red ) and oxidized (roGFP ox ) forms of the sensor via ratiometric fluorescence microscopy 24

[4] At the cellular level, free estradiol diffuses through cell membranes and binds to intracellular estrogen receptors and , which upon ligand activation dimerize and translocate to estrogen-response elements on nuclear DNA, modulating transcription of genes governing reproductive tract integrity, thermoregulation, bone metabolism, and vascular endothelial function

By focusing on the "master antioxidant," they aim to support their bodies from the inside out, rather than relying solely on topical treatments

Springer New York, New York, pp 149158