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NK cells were stained with FITC-anti-CD3 (HIT3a) and APC-anti-CD56(HCD56) antibodies (both form BioLegend) and analyzed using flow cytometry to validate their identity

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Further analysis of aged mice testes after FOXO4-DRI treatment revealed a reduction in interstitial SA--gal activity, along with decreased levels of senescence-associated proteins p53, p21, and p16