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Spheroids were formed using A431 cells and treated following a similar protocol to that used for bidimensional cultures

The complex formation of YAP/TAZ with TEAD is predicated on an autopalmitoylation reaction in TEAD [22], which is not altering TEAD's localisation but vital for its association with YAP/TAZ (Figure 1), and critically enhancing the transcriptional activity of the complex [28, 29]