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The sample was diluted to 50 mM NaCl and cleared through IEX, followed by SEC using eGFP-CRBN SEC buffer (25 mM Hepes/NaOH pH7.4, 200 mM NaCl, 2 mM TCEP)

We likewise purified Mer3 from insect cells as previously described 32 , and demonstrated that both samples were free of detectable nucleic acid contamination as determined by the ratio of the absorbance at 260 nm and 280 nm in size exclusion chromatography (Supplementary Fig

A.VasquezK.WaltenbaughC

DNA, vectors, viruses or hybrids thereof may be isolated from a variety of sources, genetically engineered, amplified, and/or expressed/ generated recombinantiy (recombinant polypeptides can be modified or immobilized to anays in accordance with the invention)