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The assay was performed according to the manufacturers protocol with a small modification

The expression of SIRT1 is downregulated, but the expressions of LncRNA AK044604 (insulin sensitivity and autophagy regulatory factor) and the activated glycogen Synthase Kinase 3 beta (GSK3) are increased in db/db mice and HG-treated immortalized mouse podocyte cells (MPCs)

Controls were treated with 1 PBS and showed 100% growth, while tests measured the percentage decrease in biofilm biomass with respect to this control

It employs the TLM to predict individual oil component toxicities and the TU mixture model to predict the whole toxicity of the oil mixture