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Osteoarthritis and cartilage 22, 344354

Part of this shift is tied to the natural decline in growth hormone production that occurs with aging

Induced cells were then collected by centrifugation at 5,000 g for 10 min at 4 C and lysed by sonication after 30-min incubation at 4 C in buffer A [20 mM sodium phosphate, pH 7.4, 500 mM NaCl, 5 mM -mercaptoethanol, 10% (v/v) glycerol]) supplemented with 1 mg/mL lysozyme (Thermo Fisher Scientific), 25 U/mL Pierce Universal Nuclease (Thermo Fisher Scientific), EDTA-free protease inhibitor Cocktail (Roche, Waltham, MA, United States) and 10 mM imidazole (Merck Life Science)

Iron overload affects the progression of OA