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Purification of GST-tagged proteins For protein expression, 400 mL bacterial cultures (DH5 or BL21 Codon Plus RIPL) were transformed with the corresponding expression plasmids, grown in LB medium supplemented with appropriate antibiotics and induced with 0.1 mM IPTG at OD 600 = 0.81.0

The most common cause of Vitamin B12 deficiency is a lack of intrinsic factor, a protein the stomach produces that is essential for B12 absorption

FSA is conducted for important metabolites such as ATP, NADPH, NO, succinate, and itaconate in normal (without turning off any pathways), glycolysis off and OXPHOS/TCA off condition