Patients were then allowed to drink clear liquids and eat light snacks 2 and 4 h after capsule ingestion, respectively
In conclusion, while metformin has shown promise in several gynecological disorders, more targeted clinical trials are necessary to establish its role in endometriosis, POF, myomas, and gynecological malignancies
After blocking with 5% BSA for 1 h, the membranes were incubated with anti--SMA (1:1,000 dilution, Abcam, USA) anti-Nrf2 (1:1,000 dilution, Proteintech Group, Rosemont, IL, USA), anti-HO-1 (1:1,000 dilution, Proteintech Group, Rosemont, IL, USA), anti-GPX4 (1:1,000 dilution, Abmart, Shanghai, China), anti-xCT (1:1,000 dilution, Shanghai, China), anti--tubulin antibodies (1:1,000 dilution, Cell Signaling Technology, Danvers, MA, USA) or anti-Lamin-B1 antibodies (1:10,000 dilution, Proteintech Group, Rosemont, IL, USA) at 4 C overnight, followed by horseradish peroxidase-linked anti-mouse (1:1,000 dilution, Cell Signaling Technology, Danvers, MA, USA) or anti-rabbit secondary antibodies (1:2,000 dilution, Cell Signaling Technology, Danvers, MA, USA) at 37 C for 1 h
Trends Cancer 7 , 790804 (2021)