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The absorbance value of the samples was then measured at 517nm, after 5 minutes of incubation at room temperature using spectrophotometer

Selective regulation of bone cell apoptosis by translational isoforms of the glucocorticoid receptor

Mol Genet Metab 136:268273

A foundational technique involves monitoring the SOD-mediated inhibition of ferricytochrome c reduction by superoxide, typically observed as a change in absorbance at 550 nm, already detailed for superoxide anion quantification (McCord and Fridovich, 1969