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Why Any Rapid Weight Loss Costs Some Muscle Muscle is metabolically expensive to maintain, so when the body is in a sustained energy deficit it will draw on both fat and lean tissue for fuel and will down-regulate the tissue it isn't being asked to use

Additionally, several factors can distort the interpretation of sex differences in drug metabolism, including genetic polymorphisms affecting enzymes like UGT, TPMT, and COMT, hormonal fluctuations, age-related changes, underlying health conditions, co-medication, and variations in study design (e.g., unbalanced sex ratios, inconsistent dosing strategies

The homogenates were centrifuged at 4 C for 20 min at 12,000 g , and the resulting supernatants were used for MDA analysis

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