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The Cu2+ ion is coordinated by the histidine imidazole nitrogen and the N-terminal amino group of glycine, forming a specific metal-peptide complex

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2448 h post-transfection, the transfected cells were identified and collected by fluorescence-activated cell sorting based on GFP fluorescence using a BD FACSAria II sorter (BD Biosciences, San Jose, CA) equipped with a GFP filter (530/30 nm)