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A marked inter-patient variability in purinergic metabolites emerged as a consistent feature of the cohort

Clearly, some kind of selectivity must be exercised if complex mixtures are to be avoided

Zhou SQ, Sakamoto K

In particular, DNA samples (50 ng) were amplified using the respective LightSNiP Assay for each SNP and Lightcycler FastStart DNA Master HybProbe Mix (Roche, Germany), according to the following PCR protocol: initial denaturation for 10 min at 95C, followed by 45 cycles of denaturation for 10 s at 95C, primer annealing for 10 s at 60C and extension for 15 s at 72C, followed by melting curve analysis to determine homozygosity for the wild-type alleles, heterozygosity, and homozygosity for the variant alleles