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Despite some earlier in vitro studies, the first evidence in intact animals suggesting MT's cardioprotective role in myocardia I/R injury was shown in a cardiac-specific MT-overexpressing transgenic mouse model 87

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in vitro NAFLD model using palmitate-treated HepG2 cells, which mimic lipid overload and metabolic stress typical of early disease

In another instance, overexpression of the Cu/Zn-SOD gene in Nicotiana tabacum plants was done to improve oxidative stress resistance, while tolerance to methyl viologen and pure cercosporin was achieved in sugar beet by transferring a Lycopersicon esculentum SOD gene (Tertivanidis et al., 2004