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Histological staining and analysis Human or mouse breast primary or lung metastatic tumor tissues were fixed, embedded in paraffin, and cut into 4-m-thick sections

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Relative abundance of H3K27me3, a repressive heterochromatic mark associated with gene silencing, was notably decreased in GNMT 8, while H3K24me1 was increased consistent with our previously mentioned global arginine and lysine methylation findings ( Figure 6F )