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The effluent was ionized via ion electrospray in negative mode on a TSQ Quantiva triple-quadrupole mass spectrometry with the following instrument settings: ion spray voltage 2500 V, sheath gas 28, ion transfer tube temperature 325, aux gas 8, and FWHM of 0.7 for both Q1/Q3 resolution

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According to the most recent diagnostic criteria for MCAS, a 200% increase over baseline levels and that is above the normal range of the assay could be considered objective evidence of MCA [3]

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