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LOXL2-DM was used as a negative control in this assay, as the LTQ generation is a self-processing reaction that requires copper binding 52,53 that is lacking in the LOXL2-DM 40

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DeGrados team 40 , which achieved precise assembly by establishing the Crick parameter equation for transmembrane helices and using RosettaMP design to obtain de novo CHAMP sequences, combined with a more rational energy scoring mechanism (Rosettas PackStat score) 45 to continuously improve the design accuracy

Clinical translation: biomarkers closest to bedside use in MASLD As the field of MASLD biomarker research progresses, several candidates are emerging as practical tools for clinical translation [77]