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For genotyping, DNA from toe clipping of littermate animals was extracted using a house-made Lysis buffer (0.1 M Tris-HCl pH 7.5, 0.1 M EDTA, 0.1 M NaCl, 1% SDS), and the PCR was performed using primers described in Supplementary Dataset 5 and the products were analyzed on an agarose gel

The leading mechanisms identified in preclinical research include: VEGFR2 Activation (New Blood Vessel Growth): BPC-157 activates VEGF Receptor 2 on blood vessel cells, triggering angiogenesis the formation of new capillaries into damaged tissue

Two other species of oyster mushroom also have substantial ERGO content: The King oyster (Pleurotus eryngii) and Pearl oyster (Pleurotus ostreatus) species

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