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& Rohani, L

This novel metabolite is formed in human plasma to a much greater extent than in the preclinical species tested (mouse, rat, dog, and cynomolgus monkey) and due to its -opioid potency may substantially contribute to the pharmacology of kratom in humans to a greater extent than in other tested species

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It incorporates key modifications, including 14E/17R mutations to stabilize an -helical segment via a salt bridge, multiple proline substitutions (25P, 28P, 29P) to reduce -sheet formation, and N-terminal C20 fatty diacid lipidation for reversible albumin binding (Kruse et al