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Proliferation assay DOHH2 cells were kept in RPMI Medium 1640 (Gibco, Cat# 11875-119) supplemented with 10% Fetal Bovine Serum (Sigma, Cat# F2442), at 37 C, 5% CO 2 in a humidified incubator

To our surprise, we observed significantly high mortality of B

Despite the strengths provided through the INNODIA study design, the representation of different centres, spread of age, autoantibody combinations and limited number of individuals with 12 month samples could have masked some underlying differences

82 While the mechanism is not fully understood, PD1/PDL1 immunotherapy and the subsequent cytotoxic tumor destruction are thought to induce a proinflammatory state that could stimulate melanocyte activity