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Superoxide Generation from Nitric Oxide synthase: Role of Cofactors and Protein Interaction

After derivatization with 10 l of 5 mM 7-diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (Sigma-Aldrich) at room temperature for 10 min, the maleimide ring was cleaved by addition of 30 l of 50 mM ammonium formate (Sigma-Aldrich) pH 4.2 at room temperature for another 30 min

Compared to non-senescent cells and young animals, aging ECs and aged mice exhibit an enhanced vulnerability in endothelial permeability and BBB dysfunction in response to hypoxia