cDNA was synthesized from 1.5 g of each total RNA samples using a random primer and reverse transcriptase enzyme (Applied Biosystems, USA)
Journal of Carcinogenesis & Mutagenesis, 5 , 1000165
The ClueGO plugin in Cytoscape was used to demonstrate the internal interconnection of ontologies and pathways involved in common DEGs with medium network specificity and showing pathways with p -value 1.5 ( 2.5 Significant shared gene detection and functional interaction Since dense interaction existed in the present large-scale network, the CytoHubba plugin in Cytoscape was applied to detect significant shared genes based on topological algorithms consisting of BottleNeck, Cluster coefficient, EPC, MCC, and MNC ( was employed to decipher the information on co-localization, co-expression, and functional characterization among these genes ( 2.6 Transcriptional and post-transcriptional analysis Considering the underlying regulations between transcription factor (TF), miRNA, and genes, significant shared gene-related TFs and miRNAs analysis was implemented in the NetworkAnalyst online tool ( based on the RegNetwork repository ( 2.7 External validation of candidate hub crosslinks To enhance authority and stringency, the significant shared genes were validated in ED- and depression-related datasets
MuSCs have served as a model system for the study of stem cell aging, 9,10 in part because they can be unequivocally identified in situ and prospectively isolated based on the expression of proteins that distinguish them from all other cell types in muscle tissue
