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Media transfer and quantification of extracellular GSH Following overnight incubation, WT, gsh2 mutant, and gsh2::GSH2 cells were washed twice with sterile water, resuspended, and transferred into 25 ml YPD broth at an OD 600 of 0.3 for an 8 h growth period

Furthermore, ATPase starts acting in reverse mode, carrying out ATP hydrolysis instead of its synthesis [181]