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To clarify whether the first N-terminal residues of BNIP3 represent the discrete structural unit that directly can activate BAX, we conducted an established in vitro BAX activity assay utilising BNIP3-peptide 1-49, other BH3-only BAX activators (BIM and PUMA) as positive controls 38 , and bovine serum albumin and the antiapoptotic BCL XL as negative controls 2

Regulation of preproinsulin mRNA stability in health and diabetes Preproinsulin mRNA has a long half-life, which is mainly regulated by a conserved polypyrimidine tract and a UUGAA-motif in its 3-UTR [13, 16]

We have included substrate activation (the Hill term on the right) and found that if we omitted this substrate activation then the cytochrome oxidase reactions did not produce enough NAPQI at high overdoses

Scaffolds were incubated with primary antibodies overnight at 4 C and with secondary antibodies for 2 h at room temperature