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In this article outlines the three phases of detoxification, explain how each phase functions, and review evidence-based strategies to support them safely

Oxidative stress is a pivotal factor in the complex pathophysiology of pain, with glutathione peroxidase 4 (GPX4) recognized as a crucial antioxidant enzyme involved in both antioxidant defense mechanisms and ferroptosis pathways

Having confirmed the glutathionylation of this protein both in vitro and in THP-1 cells, we hypothesized that it was not detected in all replicates of the array due to the low concentration present in the cell supernatants

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