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For vaccine induced protection against mycobacterial infections, CD4+ cells are vital and multiple studies have demonstrate that CD8+ cells play critical role in controlling mycobacterial infections via perforin, granzymes, and granulysin ( ex-vivo result of the IcL group is more difficult to interpret as the primed PBMCs indicated higher survival rate for MAP compared to macrophage alone or incubated with unprimed PBMCs

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The exception is when a vendor's protocol explicitly calls for it typically for peptides being prepared for immediate single-dose use rather than weekly or daily multi-dose administration