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All samples had their RNA quantified by fluorometry using the Qubit RNA HS Assay Kit (#Q32852) and the Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA)

This method has also been applied to medicinal plants such as Eastern wormwood (Artemisia scoparia), red sage ( Salvia miltiorrhiza ), motherwort ( Leonurus japonicus ), caraway ( Carum carvi ), and French lavender ( Lavandula angustifolia ) 6,7

Necropsy findings show diffuse neutrophilic infiltration and alveolar hemorrhage in the lungs, in addition to leukemia infiltration in several organs.5960 Clinically, DS presents as dyspnea, pulmonary infiltrate on X-ray, fever, pleural or pericardial effusion, acute renal failure, hypotension, weight gain and edema.53 Infectious and cardiac etiologies must be ruled out, but if suspected, treatment must be initiated promptly

Consequently, the quantity of ligand-Halo generated from the lysosomal degradation of ligand-Halo-LC3B serves as an indicator for levels of autophagy flux