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Colonies were collectively resuspended in 200 mL LB/Amp medium, and 50 mL of this suspension was subjected to plasmid DNA extraction (Midiprep Kit

The reduced imaging bold signal in associative striatum also correlated with reduced real-life motivation (Mucci et al., 2015a)

Materials and methods Cell culture Human HCC cell lines HepG2 and PLC/PRF/5 were acquired from the American Type Culture Collection (ATCC, VA, USA)

2.5 Preparation of agar hydrogel containing GSNO Two grams of agar were dissolved in 100 mL of 0.1 M phosphate buffer at pH 7.4 at 80C for 3 h with stirring