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Semax is a synthetic peptide derived from a fragment of adrenocorticotropic hormone (ACTH) and has been widely studied in laboratory research related to neurobiology, cognitive signaling pathways, and neuroprotective mechanisms

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Further, tissue sections were incubated in HRP-labeled secondary antibody (diluted to 1:3000) for 1 h at room temperature and visualized under light microscope (Lx 300, Labomed, USA)