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Introduction to Plant Oxylipin Biosynthesis and Function There are few definitive reports of the presence of arachidonic acid in higher plants, although it is present in some algae and lower plants, so plant oxylipins are octadecanoids derived from linoleic acid (18:2( n -6)) or more often linolenic acid (18:3( n -3)), released from their lipid bonds by acyl hydrolases (lipases) of various kinds

70 mL of 10 % HCl was added to the resulting concentrated solution which was then heated and stirred at a temperature of 80 to 90 0 C for 2 hours, concentrated by distillation under reduced pressure, and dissolved in a small amount of water, followed by elution on a column packed with an anion exchange resin (Amberlite IRA 410, OH " )

(B) Zoomed-in view detailing the distance between the sulfur atoms of Cys128 and Cys150