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They found that it wasnt possible to determine a clear lower limit for which any of these biomarkers can reliably indicate B12 deficiency, but that daily intakes above 4.0 g appear to have no additional benefit

However, it has been classified as GRAS (Generally Recognized As Safe) as a food additive, and is commonly used in regenerative medicine under professional supervision

Borderline is 200300 pg/mL and low or deficient B12 is defined as 100 fL) means RBCs are larger than normal and can indicate anemia (Raposo LS, 2025)

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