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The hPrdx6 (1 mg/ml) at pH 7.0 and pH 4.0 was subjected to SEC, equilibrated with 3 bed volumes of 10 mM TrisHCl buffer (pH 7.0), and 10 mM sodium acetate buffer (pH 4.0), respectively

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In this assay, oxidized glutathione, produced upon reduction of peroxide substrate by glutathione peroxidase, is then recycled back to glutathione using glutathione reductase and NADPH

To use potato juice, grate a small potato and extract its juice by squeezing it through a cloth or pressing it with your fingers