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By taking advantage of the increased 14 C atmospheric levels during the Cold War and 14 C incorporation into lipid, the group of Peter Arner and Kristy Spalding were able to calculate lipid age and turnover, demonstrating that lipolysis is defective during aging (235)

Real-time quantitative PCR analysis Total RNA was extracted from liver tissues using the TRIzol reagent (Invitrogen, USA) according to the manufacturers instructions

doi: 10.3389/fphar.2022.908772 Received 31 March 2022 Accepted 10 May 2022 Published 24 May 2022 Volume 13 - 2022 Edited by Xu Chen, Guilin Medical University, China Reviewed by Yin Lin Ge, Qingdao University, China Pengyu Chang, First Affiliated Hospital of Jilin University, China Updates Copyright 2022 Zhang, Hou, Chen, Kan, Han, Zhang and Sun

Using a probe, the surgeon tests the integrity of the soft tissues