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Description Reviews (0) Description GHK-Cu Copper complex of the tripeptide glycyl-L-histidyl-L-lysine

For metabolite detection, beads were reconstituted in 50 L of 80% methanol containing 20 M of [ 13 C 3 , 15 N]-NEM-Cysteine (Cambridge Isotope Laboratories, CNLM-38710H-0.25) and 40 M of [ 13 C 2 , 15 N]-NEM-GSH (Sigma-Aldrich, 683620), incubated on ice for 5 min, and extracts cleared by centrifugation at 17,000 g for 10 min

Symptoms of vitamin B12 deficiency can include fatigue, weakness, numbness or tingling in the hands and feet, difficulty walking, memory problems, and anemia

GST fragments with homologous arms were ligated through Exnas II (Vazyme Biotech Co., Ltd., Nanjing, China) into the linearization pcDNA3.1(+) digested by Hind III-HF (Vazyme Biotech Co., Ltd., Nanjing, China)