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Chronic alcohol exposure can lead to mitochondrial dysfunction, characterized by impaired ATP production and increased ROS generation (Hoyt et al

Standard or sample was transferred in triplicate to a 96-well plate and GSH was then derivitized by the addition of 10 mM 2,3-naphthalenedicarboxyaldehyde and the fluorescence at Ex/Em wavelength of 472/528 nm was quantified using a fluorescent plate reader (Synergy 2, BioTek)

Please note that you will need to follow a process in place in order to receive the Iron Infusion