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In line with these observations, the treatment of the murine syngeneic PCa and bladder cancer models with the JHU-083 inhibitor led to the reprogramming of the tumor-associated macrophages (TAMs) from an immunosuppressive to inflammatory state and increased TAM proliferation and glycolysis [99]

Cellular in situ LOXL2 activity assay For the gain-of-function assays, A7r5s were seeded on coverslips at 80% confluence, allowed to adhere, and serum-starved overnight

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This study also indicated that TCR signaling regulates the levels of these TNFRSF members, allowing thymocytes to respond more efficiently to GITRL, OX40L and TNF signal and thereby facilitating their differentiation into tTregs (129)