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The solution was centrifuged at 16,000 g for 2 min and the supernatant was used to measure absorbance at 530 nm

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Contribution of RhopH2 and RhopH3 to NPP The CLAG3 proteins are synthesized toward the end of the parasite asexual cell cycle, where they are stored in the rhoptry organelle in a complex, termed the RhopH complex, with two unrelated proteins named RhopH2 and RhopH3, which are encoded by single copy genes [( Plasmodium species, although the number of Clag genes varies among Plasmodium species