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Description

Immunoaffinity purification of human IgG [anti-GliT] Serum specimens (provided by the Irish Blood Transfusion Service, Dublin, Ireland according to institutional guidelines) containing high titer IgG [anti-GliT] were pooled, diluted 1 in 4 in PBS, and applied to a GliT-Sepharose affinity column (0.5 ml), prepared as per manufacturer's instructions