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Perturbing periventricular vessel ingrowth causes cortical hypoxia and impairs neurogenesis To investigate a possible requirement of angiogenesis for the switch of NPCs from expansion to neurogenesis, we studied cortical development in homozygous Gpr124 LacZ knockin null (Gpr124 KO ) embryos, which show central nervous system (CNS)restricted impairment and perturbation of angiogenesis in the forebrain and ventral spinal cord, but not in peripheral organs (Kuhnert et al , 2010

The flow-through fraction containing the Fab fragments was collected, while the Fc fragments were eluted using a high-salt buffer (2.2 mM PBS supplemented with 500 mM NaCl)