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The total GSH concentrations were estimated using an enzyme-labeled immunosorbent assay (ELISA)

Die schdlichen Wirkungen freier Radikale zu bekmpfen

In response to elevated IOP, the microglia exhibit different signs of activation, such as proliferation and migration to areas of damage, retraction, reorientation, and hyper-ramification of their processes, and the appearance of amoeboid- and rod-like microglia with macrophagic capacity, related to RGC degeneration (Ramrez et al., 2020a

Subsequently, the cells were incubated overnight with anti-vWF primary antibody, followed by staining with Alexa Fluor 594-labeled secondary antibody and DAPI for nuclear visualization