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Cu(II) binding to this site is reversible, however, characterized by slow unassisted dissociation rate and picomolar affinity ( K D = 0.90 0.09 pM), determined with NTA or fast and His-assisted dissociation and apparent subpicomolar affinity ( K D = 34.7 4.5 fM)

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Anti-oxidants such as coenzyme Q10, melatonin, and nicotinamide have been shown to mitigate oxidative stress, enhance mitochondrial function, reduce apoptosis, and im-prove the quality of oocytes and the success rates of ovarian tissue cryopreservation

The p-anisidine value measures secondary oxidation products, so the IFOS and GOED standard is less than 20 units