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Naunyn-Schmiedebergs Archives Pharmacol

For this, 50 L of beads (Pierce) were blocked with free D-Biotin (Novabiochem) (3 mg/mL prepared in alkaline water (pH 10.5)) and rocked for 1 h at RT, followed by 5 washes with 1 PBS and incubation with the cell lysates (50 L) for 30 min at 4 C with rocking to remove non-specific binding of proteins to the bead complex