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Description

Yan Y, Jia H, Wang F, Wang C, Liu S, Guo X

For cell wall laccase activity, washed cultures were added 1:100 in 100 ml of L-asparagine medium (0.1% L-asparagine, 0.1% dextrose, 3mg ml - 1 KH 2 PO 4 , 0.25 mg ml - 1 MgSO 4 7H 2 O, 1 mg ml - 1 thiamine, 5 ng ml -1 biotin) and grown for 48 h, shaking at 120 rpm

One is thiol methylation, which is catalyzed by the enzyme thiopurine S-methyltransferase (TPMT), to form the inactive metabolite methyl-6-MP (6-MeMP)