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This technique enables monitoring of cell invasion from a defined starting point in a spatiotemporal manner in normal as well as hypoxic three-dimensional culture conditions ( Figure 5B )

The MTs were initially discovered in equine renal cortex ( Figure 2 ) (Margoshes and Vallee, 1957) and the first amino acid sequences of mammalian MTs revealed that these proteins contained 6162 amino acids, of which 20 were highly conserved cysteines residues

Two selection campaigns were performed in parallel: the first on L1 treated with 1 mM DTT (L1-Lin, peptide in linear conformation) and the second on L1 treated with 1 mM DTT then 150 M CCA (L1-CCA, peptide in CCA cyclized conformation)

O., Ayinla, M