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Triggered firing in pulmonary veins initiated by in vitro autonomic nerve stimulation

2000 Oct;20(19):7311-8

Milligan G (2009) G protein-coupled receptor hetero-dimerization: contribution to pharmacology and function

Briefly, 3 g of each protein sample was mixed with 15 L of 50 mM ammonium bicarbonate (pH 7.8) (Thermo Fisher Scientific), followed by protein reduction with 2 L of 100 mM dithiothreitol (Thermo Fisher Scientific) for 30 min and protein alkylation with 5 L of 2-iodoacetamide (Thermo Fisher Scientific) for an additional 30 min at room temperature in the dark