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Nasal administration technique Using PT-141 nasal spray: Blow nose gently (clear passages) Prime pump (1-2 test sprays into air) Tilt head slightly forward (not back) Insert nozzle into nostril (don't deep) Spray while breathing in gently 2-3 sprays per nostril typically Alternate nostrils Avoid sniffing hard (keeps in nasal tissue) Wait 2-3 minutes between sprays Absorption through nasal mucosa: PT-141 absorbed across nasal lining Enters bloodstream directly Bypasses stomach/liver (like injection) But: Lower bioavailability (~30%) Hence need 2-3x higher dose Nasal spray pros/cons: When to choose nasal: Needle phobic Value convenience highly Don't mind using 2-3x more peptide Want slightly faster onset Traveling (easier to transport) When to choose injection: Want cost efficiency Comfortable with injections Prefer guaranteed absorption Limited PT-141 supply Already using other injectable peptides Managing PT-141 side effects Minimizing nausea and other reactions

Without specific data on other dosages (which I would like to obtain), these patterns suggest that a dosage regimen of hCG 250iu-350iu on an every other day (QOD) schedule would likely offer an alternative regimen for relatively steady and consistent testicular stimulation, although these conclusions are only speculative and cannot be drawn definitively from this limited data