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Brine was sampled from a fully Wiltshire compliant facility longitudinally at three timepoints, Day 0 where the brine is composed of half spent brine and half newly reconstituted brine, Day 20, where this brine was used for 20 days to cure different meats and Day 40, where the brine was deemed to be spent. The brine was sampled in late 2016 from three monitored curing containers

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doi: 10.1016/j.arcmed.2015.05.005 226 ZhouFMaiTWangZZengZShiJZhangFet al

Flow cytometry For flow cytometry, tissue was minced and incubated in collagenase 0.5 mg collagenase D (Millipore Sigma, 11088858001) in 1 mL NaCl, 30 mins, at 37C and passed through a 100 m and a 40 m strainer