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doi: 10.3390/jpm14020170

Briefly, C57Bl/6 neonatal (p4) mice harboring floxed p53 and stop-flox mCherry-luciferase were anesthetized using hypothermia and orthotopically injected with a PDGFAinternal ribosomal entry site (IRES)cyclization recombination (Cre) retrovirus (stereotaxic coordinates relative to bregma: 1 mm anterior, 1 mm lateral, 1 mm deep), resulting in tumor cells that overexpress PDGFA and mCherry-Luciferase, and have deleted p53 36