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() Research comparing NAD bioavailability supports sustained, lower-dose administration over bolus IV therapy

Different concentrations for methionine and folate supplementation were based on previous studies 15,19

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Cells were plated at 10 6 cells/well and cultured overnight (5% CO 2 , 37 C), then stimulated for 24 h at either a low (0.25 mM) or high (4.25 mM) concentration of the 33-mer peptide, or its Clc-derived fragments (9-mer, 7-mer and 3-mer