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El-Sayed ASA, Shindia AA, Ali GS, Yassin MA, Hussein H, Awad SA, et al

The original FRET PKA-based cAMP biosensor uses fluorescein (donor) and rhodamine (acceptor) to measure the decrease in the FRET signal due to elevation in intracellular cAMP, leading to dissociation of the regulatory subunit of PKA from its catalytic segment (Mongillo et al., 2004)